Advancing the understanding of physiological and neuronal processes with 3D technology. Our high-speed fluorescence imaging allows for the gentle long-term observation of living organisms, while also accelerating data collection on large samples.
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ZEISS has introduced a new microscopy technology called Lightfield 4D, which is integrated into the new ZEISS LSM 910 and LSM 990 confocal microscope systems. This innovative imaging mode allows for instant volumetric high-speed imaging, providing unprecedented temporal resolution for observing dynamic processes in living organisms. It is especially beneficial for research in neurosciences, cancer, developmental biology, and plant sciences.
The new technology provides a solution for 4D imaging, which combines volume and time to better understand biological processes. Conventional optical sectioning techniques that rely on sequential image acquisition to create Z-stack images have long faced limitations, such as time delays and compromised spatiotemporal accuracy of data. ZEISS Lightfield 4D overcomes these challenges by capturing entire 3D data sets at a precise moment in time, eliminating time delays within a captured volume.
Revolutionizing microscopic imaging: Capturing comprehensive 3D information in real-time
The ZEISS Lightfield 4D utilizes a micro lens array to generate 37 individual images simultaneously, allowing for the creation of Z-stacks through advanced deconvolution-based processing. This innovative approach achieves an impressive rate of up to 80 volume Z-stacks per second, providing insights into rapid biological events such as physiological and neuronal processes.
Enhancing understanding of biology through fast and gentle imaging
With just one illumination event, a full Z-stack is captured, minimizing light exposure and phototoxicity. This enables long-term imaging of whole living organisms at high temporal resolution, tracking biological processes over extended periods. ZEISS Lightfield 4D’s exceptional speed allows for the rapid capture of multi-color volumes of large samples, boosting experiment productivity in a range of applications.
Dr. Bernhard Zimmermann, Head of Life Sciences Microscopy at ZEISS, believes that the ZEISS Lightfield 4D system, which combines volume and time, represents a significant advancement in 4D imaging. He is confident that this innovative system will redefine the imaging landscape, allowing researchers to capture biological processes in real-time and gain unprecedented insights into the dynamic nature of life.
Introducing the latest LSM platforms, now with ZEISS Lightfield 4D technology
By combining the incredible imaging flexibility of laser scanning microscopes (LSMs) with the gentle and instant volume imaging capabilities of ZEISS Lightfield 4D, previously impossible experiments are now possible, pushing scientific research to new frontiers.
We are excited to announce the release of the upgraded versions of ZEISS LSM 910 and LSM 990, in conjunction with the launch of Lightfield 4D. These enhanced systems feature new high-bandwidth electronics to support beampath technology, allowing for exceptional light preservation, visualization of high dynamic ranges, and a broad wavelength bandwidth. Furthermore, all Airyscan Multiplex modes now include image quality improvements with ZEISS Airyscan jDCV. Researchers can also take advantage of the Microscopy Copilot, a personal AI assistant, to explore new possibilities for their imaging experiments interactively.
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